50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine.
-80 deg C
Amino acids A2 – M355 (end) of human KLF2.
Residue A232 of the fusion protein is equivalent to A2 of the native enzyme. The GST tag is located at residues 1 – 220.
The protein has the following amino acid substitution:
L – P, where L104, is residue P334 is the fusion protein.
This change is a defined snp.
PreScission (LEVLFQGP) residues 221 – 228
BamH1 and EcoR1 site of pGEX6P-1
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