Recombinant Protein - KLF2 (2 - 355)

Name: KLF2 (2 - 355)
Price per aliquot (100µg): £110.00
Long Name:
Kruppel like factor 2
Catalog Number (DU Number):
DU3100
Accession:
NM_016270.3
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH Sepharose
Calculated molecular mass:
Mono-Isotopic Mass: 64, 055.35 daltons
Average Mass: 64, 096.51 daltons
Purity:
>75 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine.
Storage Temperature:
-80 deg C
Theoretical pI:
8.12
Native Sequence:
Amino acids A2 – M355 (end) of human KLF2. Residue A232 of the fusion protein is equivalent to A2 of the native enzyme. The GST tag is located at residues 1 – 220. The protein has the following amino acid substitution: L – P, where L104, is residue P334 is the fusion protein. This change is a defined snp.
Protease Cleavage:
PreScission (LEVLFQGP) residues 221 – 228
Cloning Sites:
BamH1 and EcoR1 site of pGEX6P-1

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