Recombinant Protein - HSP 27 (2 - 205)

Name: HSP 27 (2 - 205)
Price per aliquot (100µg): £110.00
Catalog Number (DU Number):
DU808
Accession:
NM_001540
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH Sepharose
Enzymatic Assay Format:
Substrate for MAPKAP-K2
Calculated molecular mass:
Mono-Isotopic Mass: 49, 444.09 daltons
Average Mass: 49, 475.50 daltons
Purity:
90 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine.
Storage Temperature:
–70 °C
Theoretical pI:
5.84
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids T2 – K205 (end) of human HSP27. Residue T232 of the fusion protein is equivalent to T2 of the native protein. The GST tag is located at residues 1 - 220
Protease Cleavage:
PreScission (LEVLFQGPL) residues 221 - 229
Cloning Sites:
BamH1 and EcoR1 of pGEX-6P-1

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