Name: hnRNP K isoform b S116A (2 - 463)
Price per aliquot (100µg): £110.00
Catalog Number (DU Number):
Enzymatic Assay Format:
Control substrate for ERK1 or ERK2
Calculated molecular mass:
Mono-Isotopic Mass: 77, 977.17 daltons
Average Mass: 78, 026.63 daltons
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine.
Amino acids E2 – F463 (end) of human hnRNP K isoform b.
Residue E235 of fusion protein is equilivalent to E2 of the native enzyme. The GST tag is located at residues 1 – 220.
The protein has a S116A mutation, where S116 which is phosphorylated by ERK1 / ERK2 has been changed to A. Residue S116 is equilivalent to residue A349 of the fusion protein.
PreScission (LEVLFQGPL) residues 221 - 229
EcoR1 and Not1 site of pGex6P-1