Recombinant Protein - hnRNP K isoform b (2 - 463)

Name: hnRNP K isoform b (2 - 463)
Price per aliquot (100µg): £110.00
Catalog Number (DU Number):
DU4040
Accession:
AAH14980
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH Sepharose
Enzymatic Assay Format:
Substrate for ERK1 or ERK2
Calculated molecular mass:
Mono-Isotopic Mass: 77, 993.16 daltons
Average Mass: 78, 042.63 daltons
Purity:
90 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine.
Storage Temperature:
-70 °C
Theoretical pI:
5.47
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids E2 – F463 (end) of human hnRNP K isoform b. Residue E235 of fusion protein is equilivalent to E2 of the native enzyme. The GST tag is located at residues 1 – 220.
Protease Cleavage:
PreScission (LEVLFQGPL) residues 221 - 229
Cloning Sites:
EcoR1 and Not1 site of pGex6P-1

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