Recombinant Protein - hnRNP F (2 - 415)

Name: hnRNP F (2 - 415)
Price per aliquot (100µg): £110.00
Synonyms:
Heterogeneous nuclear ribonucleoprotein F, Nucleolin-like protein mcs94-1
Catalog Number (DU Number):
DU1895
Accession:
P52597.3
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH Sepharose
Calculated molecular mass:
Mono-Isotopic Mass: 72, 318.45 daltons
Average Mass: 72, 364.83 daltons
Purity:
>80 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-70 °C
Theoretical PI:
5.49
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids M2 – D415 (end) of human hnRNP F. Residue M232 of the fusion protein is equivalent to M2 of the native enzyme. The GST tag is located at residues 1 – 220.
Protease Cleavage:
PreScission (LEVLFQGP) residues 221 - 228
Cloning Sites:
BamH1 and Not1 sites of pGEX6P-1

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