50 mM Tris-HCl pH 7.5, 50 % glycerol, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine.
Gel Information :
Gel Image 1:
Amino acids G3 – A188 of mouse hnRNP A3.
[Full length protein ends at residue F357]
Residue G232 of the fusion protein is equivalent to G3 of the native protein. The GST tag is located at residues 1 - 220
PreScission (LEVLFQGPL) residues 221 - 229
BamH1 and EcoR1 of pGEX-6P-1
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