Recombinant Protein - GRP1 PH domain (241 - 399)

Name: GRP1 PH domain (241 - 399)
Price per aliquot (100µg): £110.00
Catalog Number (DU Number):
DU5047
Accession:
BC035296
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH Sepharose
Enzymatic Assay Format:
Sensor for PIP3
Calculated molecular mass:
Mono-Isotopic Mass: 45, 541.19 daltons
Average Mass: 45, 570.47 daltons
Purity:
90 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 10 % glycerol, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.2 mM PMSF, 1 mM Benzamidine.
Storage Temperature:
–20 °C
Theoretical PI:
7.11
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids E241 – K399 (end) of mouse GRP1. Residue E232 of the fusion protein is equivalent to E241 of the native protein. The GST tag is located at residues 1 - 220
Protease Cleavage:
Thrombin (LVPRGS) residues 221 - 226
Cloning Sites:
EcoR1 of pGEX4T-3

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