Recombinant Protein - Green Fluorescent Protein (2 - 238)

Name: Green Fluorescent Protein (2 - 238)
Price per aliquot (100µg): £110.00
Catalog Number (DU Number):
DU1574
Accession:
M62653
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH Sepharose
Calculated molecular mass:
Mono-Isotopic Mass: 53, 600.08 daltons
Average Mass: 53, 634.45 daltons
Purity:
> 95 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 50 % glycerol, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine.
Storage Temperature:
–20 °C
Theoretical PI:
5.65
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids S2 – K238 (end) of Aequorea victoria Green Fluorescent Protein. Residue S231 of the fusion protein is equivalent to S2 of the native protein. The GST tag is located at residues 1 – 220. The following amino acid substitutions are present: F – L, where F64 of the native sequence is L295 of the fusion protein. S – T, where S65 of the native sequence is T296 of the fusion protein. H – L, where H231 of the native sequence is L462 of the fusion protein.
Protease Cleavage:
PreScission (LEVLFQGPL) residues 221 - 229
Cloning Sites:
BamHI and EcoRI sites of pGEX-6P-1

Do you need any help? Please get in touch and we’ll be happy to lend a hand.