Recombinant Protein - EWS (2 - 656)

Name: EWS (2 - 656)
Price per aliquot (100µg): £110.00
Catalog Number (DU Number):
DU1883
Accession:
NP_005234
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH Sepharose
Enzymatic Assay Format:
Substrate for ERK and JNK
Calculated molecular mass:
Mono-Isotopic Mass: 95, 484.46 daltons
Average Mass: 95, 544.62 daltons
Purity:
> 85 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine.
Storage Temperature:
–70 °C
Theoretical PI:
8.79
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids A2 – Y656 of human EWS. Residue A234 of the fusion protein is equivalent to A2 of the native protein. The GST tag is located at residues 1 – 220.
Protease Cleavage:
PreScission (LEVLFQGP) residues 221 - 228
Cloning Sites:
Cloning sites Nucleotide sequence of insert EcoR1 and SalI sites of pGEX-6P-1

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