Recombinant Protein - eIF4E (2 - 217)

Name: eIF4E (2 - 217)
Price per aliquot (100µg): £110.00
Catalog Number (DU Number):
DU1129
Accession:
NM_001968
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH Sepharose
Enzymatic Assay Format:
Substrate for MNK2 alpha
Calculated molecular mass:
Mono-Isotopic Mass: 51, 757.11 daltons
Average Mass: 51, 790.23 daltons
Purity:
> 95 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 50 % glycerol, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2 mercaptoethanol, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine.
Storage Temperature:
–20 °C
Theoretical PI:
5.76
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids A2 – V217 (end) of human eIF4E. Residue A232 of the fusion protein is equivalent to A2 of the native protein. The GST tag is located at residues 1 – 220
Protease Cleavage:
PreScission (LEVLFQGPL) residues 221 - 229
Cloning Sites:
BamHI and EcoRI sites of pGEX 6P-1

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