FESTIVE CLOSURE

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Recombinant Protein - DAZAP T315A (2 - 407)

Name: DAZAP T315A (2 - 407)
Price per aliquot (100µg): £100.00
Synonyms:
DAZ associated protein 1
Catalog Number (DU Number):
DU3218
Accession:
NM_018959.3
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH Sepharose
Calculated molecular mass:
Mono-Isotopic Mass: 70, 001.64 daltons
Average Mass: 70, 046.34 daltons
Purity:
>80 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-70 °C
Theoretical pI:
6.87
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids N2 – R407 (end) of human DAZAP. Residue N232 of the fusion protein is equivalent to N2 of the native enzyme. The GST tag is located at residues 1 – 220. The protein has a T315A mutation. Residue T315 is equivalent to residue A545 of the fusion protein.
Protease Cleavage:
PreScission (LEVLFQGP) residues 221 - 228
Cloning Sites:
BamH1 sites of pGEX6P-1

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