50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Gel Information :
Gel Image 1:
Amino acids S2 – S147 (end) of human CRHSP 24.
Residue S247 of the fusion protein is equivalent to E2 of the native enzyme. The GST tag is located at residues 1 – 220 and the HA tag is located at residues 238 – 246.
PreScission (LEVLFQGPL) residues 221 - 229
EcoR1 and Sal1site of pGEX 6P-1
Have a Question?
If you would like any assistance with your order, please don’t hesitate to get in touch.