50 mM Tris/HCl pH 7.5, 50 % glycerol, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine.
Gel Information :
Gel Image 1:
Amino acids M1 – A283 of rat CREB.
[Full length protein ends at residue D341]
Residue M229 of the fusion protein is equilivalent to M1 of the native protein. The GST tag is located at residues 1 – 220. The following amino acid is present after the CREB sequence, V, at residue 512.
The following amino acid substitution is present:
D – E, where D4 of the native sequence is E232 of the fusion protein
Thrombin (LVPRGS) residues 221 - 226
Sma1 and Xba1 of pGEX 2T
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