Recombinant Protein - CREB (1 - 283)

Name: CREB (1 - 283)
Price per aliquot (100µg): £100.00
Synonyms:
cAMP responsive element binding protein 1, CREB1
Catalog Number (DU Number):
DU1570
Accession:
NM_031017
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH Sepharose
Calculated molecular mass:
Mono-Isotopic Mass: 56, 100.27 daltons
Average Mass: 56, 135.60 daltons
Purity:
75 %
Storage Buffer:
50 mM Tris/HCl pH 7.5, 50 % glycerol, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine.
Storage Temperature:
–20 °C
Theoretical pI:
5.05
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids M1 – A283 of rat CREB. [Full length protein ends at residue D341] Residue M229 of the fusion protein is equilivalent to M1 of the native protein. The GST tag is located at residues 1 – 220. The following amino acid is present after the CREB sequence, V, at residue 512. The following amino acid substitution is present: D – E, where D4 of the native sequence is E232 of the fusion protein
Protease Cleavage:
Thrombin (LVPRGS) residues 221 - 226
Cloning Sites:
Sma1 and Xba1 of pGEX 2T

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