Recombinant Protein - Cofilin 2 [S3A] (1 - 166)

Name: Cofilin 2 [S3A] (1 - 166)
Price per aliquot (100µg): £100.00
Catalog Number (DU Number):
DU428
Accession:
NM_021914
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH Sepharose
Enzymatic Assay Format:
Control substrate for LIMK1
Calculated molecular mass:
Mono-Isotopic Mass: 45, 515.49 daltons
Average Mass: 45, 544.78 daltons
Purity:
95 %
Storage Buffer:
50 mM Tris/HCl pH 7.5, 50 % glycerol, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine.
Storage Temperature:
–20 °C
Theoretical pI:
6.07
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids M1 – L166 (end) of human Cofilin 2. Residue M232 of the fusion protein is equivalent to M1 of the native protein. The protein has a S3A mutation, where S3 which is phosphorylated by LIMK1 has been changed to A. Residue S3 is equilivalent to residue A234 of the fusion protein. The GST tag is located at residues 1 - 220
Protease Cleavage:
PreScission (LEVLFQGPL) residues 221 - 229
Cloning Sites:
BamH1 and Sal1 of pGEX-6P-1

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