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Recombinant Protein - c-MYC (2 - 439)

Name: c-MYC (2 - 439)
Price per aliquot (100µg): £110.00
Catalog Number (DU Number):
DU818
Accession:
NM_008351
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved

C-Term
6His
Uncleaved
Purification Method:
GSH Sepharose
Enzymatic Substrate:
Substrate for GSK3
Calculated molecular mass:
Mono-Isotopic Mass: 76, 271.90 daltons
Average Mass: 76, 319.90 daltons
Protein Activity:
Constitutively Active
Purity:
70 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-80 °C
Theoretical PI:
5.68
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids P2 – A439 (end) of human c-MYC. Residue P232 of the fusion protein is equivalent to P2 of the native enzyme. The GST tag is located at residues 1 – 220 and His6 tag at residues 670 – 675.
Protease Cleavage:
PreScission (LEVLFQGPL) residues 221 - 229
Cloning Sites:
BamHI and EcoR1site of pGEX 6P-1

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