50 mM Tris-HCl pH 7.5, 50 % glycerol, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine.
Gel Information :
Gel Image 1:
Amino acids M1 – F331 (end) of human c-Jun.
Residue M419 of the fusion protein is equivalent to M1 of human c-Jun. The MBP tag is located at residues 1 – 408 and the His(6) tag is located at residues 750 – 755.
Factor Xa (IEGR) residues 409 - 412
BamH1 sites of pMal2px
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