FESTIVE CLOSURE

Festive Closure

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Recombinant Protein - c-Jun (1 - 331)

Name: c-Jun (1 - 331)
Price per aliquot (100µg): £100.00
Catalog Number (DU Number):
DU968
Accession:
J04111
Expression:
bacteria
Tag and Terminus:
N-Term
MBP
Uncleaved

C-Term
6His
Uncleaved
Purification Method:
Amylose agarose
Enzymatic Substrate:
Substrate for JNK
Calculated molecular mass:
Mono-Isotopic Mass: 82, 080.76 daltons
Average Mass: 82, 132.18 daltons
Purity:
>85 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 50 % glycerol, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine.
Storage Temperature:
-20 °C
Theoretical pI:
6.53
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids M1 – F331 (end) of human c-Jun. Residue M419 of the fusion protein is equivalent to M1 of human c-Jun. The MBP tag is located at residues 1 – 408 and the His(6) tag is located at residues 750 – 755.
Protease Cleavage:
Factor Xa (IEGR) residues 409 - 412
Cloning Sites:
BamH1 sites of pMal2px

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