FESTIVE CLOSURE

Festive Closure

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Recombinant Protein - CAPZIP (2 - 412)(p70 MP)

Name: CAPZIP (2 - 412)(p70 MP)
Price per aliquot (100µg): £100.00
Catalog Number (DU Number):
DU396
Accession:
AAH25872
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH Sepharose
Enzymatic Assay Format:
Substrate for MAPKAP-K2
Calculated molecular mass:
Mono-Isotopic Mass: 70,763.03
Average Mass: 70,807.13
Protein Activity:
Constitutively Active
Purity:
90 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-80 °C
Theoretical pI:
5.25
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids E2 – M412 (end) of mouse CAPZIP. Residue E232 of the fusion protein is equivalent to E2 of the native enzyme. The GST tag is located at residues 1 – 220.
Protease Cleavage:
PreScission (LEVLFQGPL) residues 221 - 229
Cloning Sites:
BamHI and EcoR1site of pGEX 6P-1

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