FESTIVE CLOSURE

Festive Closure

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Recombinant Protein - ATF2 (19 - 96)

Name: ATF2 (19 - 96)
Price per aliquot (100µg): £100.00
Catalog Number (DU Number):
DU1787
Accession:
NM_001880
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH Sepharose
Enzymatic Assay Format:
Substrate for JNK1, JNK2 and JNK3
Enzymatic Substrate:
Substrate for JNK1, JNK2 and JNK3
Calculated molecular mass:
Mono-Isotopic Mass: 36,458.27
Average Mass: 36,482.05
Purity:
> 95 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 50 % glycerol, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine.
Storage Temperature:
–20 °C
Theoretical pI:
5.44
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids M19 – F96 of human ATF2. [Full length protein ends at residue S505]; Residue M236 of the fusion protein is equivalent to M19 of the native protein. The GST tag is located at residues 1 – 220. The following sequence is present after the ATF2 sequence, LEID, residues 314 - 317.
Protease Cleavage:
Thrombin (LVPRGS) residues 221 - 226
Cloning Sites:
BamHI and EcoRI sites of pGEX-4T-1

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