50 mM Tris-HCl pH 7.5, 50 % glycerol, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine.
Gel Information :
Gel Image 1:
Amino acids M19 – F96 of human ATF2. [Full length protein ends at residue S505]; Residue M236 of the fusion protein is equivalent to M19 of the native protein. The GST tag is located at residues 1 – 220. The following sequence is present after the ATF2 sequence, LEID, residues 314 - 317.
Thrombin (LVPRGS) residues 221 - 226
BamHI and EcoRI sites of pGEX-4T-1
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