COVID-19 CLOSURE

COVID-19 Closure

Due to the current situation with Covid 19 this facility is currently closed and all staff are working from home. Although we will be checking emails regularly, there may be a longer delay than usual in replying.

Recombinant Protein - APC [1484 - 1528]

Name: APC [1484 - 1528]
Price per aliquot (100µg): £110.00
Synonyms:
ANAPC4
Catalog Number (DU Number):
DU5128
Accession:
NM_000038.5
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH Sepharose
Calculated molecular mass:
Mono-Isotopic Mass: 31,685.00 daltons
Average Mass: 31,705.62 daltons
Purity:
>80 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-70 °C
Theoretical PI:
5.16
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids L1484 – V1528 (end residue is V2843) of human APC. Residue L232 of the fusion protein is equivalent to L1484 of the native enzyme. The GST tag is located at residues 1 – 220.
Protease Cleavage:
PreScission (LEVLFQGP) residues 221 - 228
Cloning Sites:
BamH1 and EcoR1 sites of pGEX6P-1

Do you need any help? Please get in touch and we’ll be happy to lend a hand.