FESTIVE CLOSURE

Festive Closure

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Recombinant Protein - APC [1484 - 1528]

Name: APC [1484 - 1528]
Price per aliquot (100µg): £100.00
Synonyms:
ANAPC4
Catalog Number (DU Number):
DU5128
Accession:
NM_000038.5
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH Sepharose
Calculated molecular mass:
Mono-Isotopic Mass: 31,685.00 daltons
Average Mass: 31,705.62 daltons
Purity:
>80 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-70 °C
Theoretical pI:
5.16
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids L1484 – V1528 (end residue is V2843) of human APC. Residue L232 of the fusion protein is equivalent to L1484 of the native enzyme. The GST tag is located at residues 1 – 220.
Protease Cleavage:
PreScission (LEVLFQGP) residues 221 - 228
Cloning Sites:
BamH1 and EcoR1 sites of pGEX6P-1

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