Recombinant Protein - 14-3-3 zeta (1 - 245)

Name: 14-3-3 zeta (1 - 245)
Price per aliquot (100µg): £100.00
Catalog Number (DU Number):
DU8266
Accession:
NM_145690
Expression:
bacteria
Tag and Terminus:
N-Term
6His
Uncleaved
Purification Method:
Ni2+-NTA agarose
Calculated molecular mass:
Mono-Isotopic Mass: 31, 269.29
Average Mass: 31, 289.00
Purity:
>85 %
Storage Buffer:
25 mM Hepes pH 7.5, 50 % glycerol, 1 mM DTT, 0.2 mM PMSF, 1 mM Benzamidine.
Storage Temperature:
–20 °C
Theoretical pI:
5.23
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids M1 – N245 (end) of human 14-3-3 zeta. Residue M35 of the fusion protein is equivalent to M1 of the native protein. The His(6) tag is located at residues 5 – 10.
Protease Cleavage:
Thrombin (LVPRGS) between residues 14 - 19
Cloning Sites:
BamH1 and Not1 of pET 28a

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