FESTIVE CLOSURE

Festive Closure

We will be closed between 21st December to 4th January. Orders may be placed during this time but will not be shipped until January 2018. Seasons Greetings to all our customers.

Recombinant Protein - 14-3-3 gamma (1 - 247)

Name: 14-3-3 gamma (1 - 247)
Price per aliquot (100µg): £100.00
Catalog Number (DU Number):
DU8262
Accession:
BC020963
Expression:
bacteria
Tag and Terminus:
N-Term
6His
Uncleaved
Purification Method:
Ni2+-NTA agarose
Calculated molecular mass:
Mono-Isotopic Mass: 31, 826.47
Average Mass: 31, 846.49
Purity:
>85 %
Storage Buffer:
25 mM Hepes pH 7.5, 50 % glycerol, 1 mM DTT, 0.2 mM PMSF, 1 mM Benzamidine.
Storage Temperature:
–20 °C
Theoretical pI:
5.34
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids M1 – N247 (end) of human 14-3-3 gamma. Residue M35 of the fusion protein is equivalent to M1 of the native protein. The His(6) tag is located at residues 5 – 10.
Protease Cleavage:
Thrombin (LVPRGS) between residues 14 - 19
Cloning Sites:
BamH1 and Not1 of pET 28a

Do you need any help? Please get in touch and we’ll be happy to lend a hand.