Recombinant Protein - ILKAP (2 - 392)

Name: ILKAP (2 - 392)
Price per aliquot (100µg): £110.00
Long Name:
ILK associated serine/threonine phosphatase
Synonyms:
integrin-linked kinase-associated serine/threonine phosphatase, PPM1O, FLJ10181, DKFZP434J2031, protein phosphatase, Mg2+/Mn2+ dependent 1O, Integrin Linked Kinase Associated Phosphatase 2 C
Catalog Number (DU Number):
DU1365
Accession:
NM_030768
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH Sepharose
Enzymatic Assay Format:
Standard phosphatase assay
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 2 mM MnCl2, 0.03 % Brij 35, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol
Enzymatic Substrate:
6 μM 32P labelled casein (phosphorylated by PKA)
Calculated molecular mass:
Mono-Isotopic Mass: 69, 555.36 daltons
Average Mass: 69, 599.5 daltons
Protein Activity:
Constitutively Active
Purity:
>80 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 2 mM MnCl2, 0.03 % Brij 35, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 50 % glycerol, 1 mM benzamidine and 0.1 mM PMSF
Storage Temperature:
-20 °C
Theoretical PI:
6.19
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids D2 – H392 (end) of human ILKAP. Residue D236 of the fusion protein is equivalent to D2 of the native enzyme. The GST tag is located at residues 1 - 220.
Protease Cleavage:
PreScission (LEVLFQGPL) at residues 221 - 229
Cloning Sites:
BamH1 and NotI sites of pGEX-6P-1

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