FESTIVE CLOSURE

Festive Closure

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Recombinant Protein - DGK beta (1 - 804)

Name: DGK beta (1 - 804)
Price per aliquot (100µg): £100.00
Synonyms:
DGKB
Catalog Number (DU Number):
DU9153
Accession:
NM_0040802
Expression:
baculovirus
Tag and Terminus:
N-Term
6His
Uncleaved
Purification Method:
Ni2+-NTA agarose
Enzymatic Assay Format:
ADP Glo
Enzymatic Buffer:
20 mM Tris-HCl, 67 mM KCl, 1 mM DTT, 0.05 mg/ml BSA, 10 mM MgCl2
Enzymatic Substrate:
Diacylglycerol Final concentration: 0.1 mM
Calculated molecular mass:
Mono-Isotopic Mass: 95, 295.55 daltons
Average Mass: 95, 357.49 daltons
Protein Activity:
Constitutively Active
Purity:
>80 %
Storage Buffer:
50 mM HEPES pH 7.0, 150 mM NaCl, 20 % glycerol, 5 mM DTT
Storage Temperature:
-70 °C
Theoretical pI:
7.70
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids M1 – E804 (end) of human DGK beta isoform 1. Residue M41 of the fusion protein is equivalent to M1 of the native enzyme. The His(6) tag is located at residues 5 – 10.
Protease Cleavage:
rTEV (ENLYFQG) residues 18 – 24
Cloning Sites:
Spe1 and Kpn1 of pFastBac HTc

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