Recombinant Protein - Choline Kinase Alpha (1 - 457)

Name: Choline Kinase Alpha (1 - 457)
Price per aliquot (100µg): £100.00
Synonyms:
CHKA
Catalog Number (DU Number):
DU14189
Accession:
NM_001277.2
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH Sepharose
Enzymatic Assay Format:
ADP Glo
Enzymatic Buffer:
12.5 mM glycine-NaOH pH 8.5, 50 mM KCl, 2.5 mM MgCl2
Enzymatic Substrate:
Choline chloride; Final concentration: 0.2 mM
Calculated molecular mass:
Mono-Isotopic Mass: 79,022.13
Average Mass: 79,073.21
Protein Activity:
Constitutively Active
Purity:
>75 %
Storage Buffer:
25 mM glycine-NaOH pH 8.5, 67 mM KCl, 2 mM EDTA, 270 mM Sucrose, 0.1 % Bmercaptoethanol 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-70 °C [Long term stability to be determined]
Theoretical pI:
5.98
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids M1 – V457 (end) of human Choline Kinase Alpha. Residue M232 of the fusion protein is equivalent to M1 of the native enzyme. The GST tag is located at residues 1 – 220.
Protease Cleavage:
PreScission (LEVLFQGP) residues 221 - 228
Cloning Sites:
BamH1 and Not1site of pGEX 6P-1

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