Recombinant Protein - SRPK1 (2 - 655)

Name: SRPK1 (2 - 655)
Price per aliquot (100µg): £110.00
Long Name:
SRSF protein kinase 1
Synonyms:
SFRSK1, SR protein kinase 1, serine/arginine-rich splicing factor kinase 1
Catalog Number (DU Number):
DU967
Accession:
NM_003137
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH-Agarose
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1mM EGTA, 10 mM DTT, 10 mM MgAc
Enzymatic Substrate:
RSRSRSRSRSRSRSR residues 204 – 218 of human ASF-1/SF-2
Calculated molecular mass:
Mono-Isotopic Mass: 100, 854.88 daltons
Average Mass: 100, 918.62 daltons
Protein Activity:
Constitutively Active
Purity:
>75 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-80 deg C
Theoretical PI:
5.79
Native Sequence:
Amino acids E2 – S655 (end) of human SRPK1. Residue E2 of the fusion protein is equilivalent to E232 of the native enzyme. The GST tag is located at residues 1 – 220.
Protease Cleavage:
PreScission (LEVLFQGP) residues 221 - 228
Cloning Sites:
BamH1 and EcoR1 sites of pGEX6P-1

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