Recombinant Protein - PKB alpha (AKT1)  [S473D] (118 - 480) *

Name: PKB alpha (AKT1)  [S473D] (118 - 480) *
Price per aliquot (100µg): £110.00
Catalog Number (DU Number):
DU1850
Accession:
BC000479
Expression:
baculovirus
Tag and Terminus:
N-Term
6His
Uncleaved
Purification Method:
Ni2+-NTA agarose
Enzymatic Assay Format:
Standard filter binding assay
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1 % 2mercaptoethanol, 0.1 mM EGTA, 10 mM MgAc
Enzymatic Substrate:
Crosstide [GRPRTSSFAEG] Final concentration: 30 μM
Calculated molecular mass:
Mono-Isotopic Mass: 45, 103.18 daltons
Average Mass: 45, 132.24 daltons
Purity:
>80 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.2 mM PMSF, 1 mM Benzamidine.
Storage Temperature:
-70 °C
Theoretical pI:
5.74
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids M118 - A480 (end) of human PKB alpha. Residue M29 of the fusion protein is equivalent to M118 of the native enzyme. The enzyme has a S473D mutation to mimic phosphorylation of the PDK2 site. Residue S473 is equilivalent to D384 of the fusion protein. The His(6) tag is located at residues 5 - 10.
Protease Cleavage:
rTEV (ENLYFQG) residues 18 – 24
Cloning Sites:
BamHI and KpnI sites of pFastBAC HTb.

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