Name: MKK1 (MEK) (1 - 393)
Price per aliquot (100µg): £110.00
Catalog Number (DU Number):
GSH Sepharose followed by Ni2+-NTA agarose
Enzymatic Assay Format:
Two step assay in which MKK1 activates unactive MAPK2/ERK2 [DU 650 or DU 1844]. Activity of MAPK2/ERK2 is then assayed against myelin basic protein as substrate (final concentration of 0.3 mg/ml), in the standard filter binding assay.
50 mM Tris-HCl pH 7.5, 0.1 % 2-mercaptoethanol, 0.1 mM EGTA, 10 mM MgAc
Calculated molecular mass:
Mono-Isotopic Mass: 70, 909.23 daltons
Average Mass: 70, 954.83 daltons
50 mM Tris-HCl pH 7.5, 150 mM NaCl, 270 mM sucrose, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Amino acids P2 – V393 (end) of human MEK1.
Residue P232 of the fusion protein is equivalent to P2 of the native enzyme. The GST tag is located at residues 1 – 220 and the His(6) is located at residues 624-629.
Prescission site (LEVLFQGPL) at residues 221 - 229
BamH1 site of pGEX-6P-1