Name: MARK1 (2 - 795) *
Price per aliquot (100µg): £110.00
Catalog Number (DU Number):
Enzymatic Assay Format:
Standard filter binding assay
50 mM Tris-HCl pH 7.5, 0.1mM EGTA, 0.1 % 2-mercaptoethanol, 10 mM MgAc
AMARA peptide [AMARAASAAALARRR] Final concentration: 300 μM
Calculated molecular mass:
Mono-Isotopic Mass: 92, 529.72 daltons
Average Mass: 92, 587.41 daltons
50 mM Tris-HCl pH 7.5, 270 mM sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine.
-70 °C [Long term stability to be determined]
Amino acids S2 – L795 (end) of human MARK1.
Residue S31 of fusion protein is equilivalent to S2 of the native enzyme. The His(6) tag is located at residues 5 – 10.
The following amino acid substitutions are present:
V – E, where V16 of the native enzyme is E45 of the fusion protein T – S, where T20 of the native enzyme is S49 of the fusion protein
rTEV (ENLYFQG) residues 18 - 24
EcoR1 site of pFastBAC HTa