Recombinant Protein - MARK1 (2 - 795) *

Name: MARK1 (2 - 795) *
Price per aliquot (100µg): £110.00
Catalog Number (DU Number):
DU1272
Accession:
AF154845
Expression:
baculovirus
Tag and Terminus:
N-Term
6His
Uncleaved
Purification Method:
Ni2+-NTA agarose
Enzymatic Assay Format:
Standard filter binding assay
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1mM EGTA, 0.1 % 2-mercaptoethanol, 10 mM MgAc
Enzymatic Substrate:
AMARA peptide [AMARAASAAALARRR] Final concentration: 300 μM
Calculated molecular mass:
Mono-Isotopic Mass: 92, 529.72 daltons
Average Mass: 92, 587.41 daltons
Protein Activity:
Constitutively Active
Purity:
75 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine.
Storage Temperature:
-70 °C [Long term stability to be determined]
Theoretical PI:
9.24
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids S2 – L795 (end) of human MARK1. Residue S31 of fusion protein is equilivalent to S2 of the native enzyme. The His(6) tag is located at residues 5 – 10. The following amino acid substitutions are present: V – E, where V16 of the native enzyme is E45 of the fusion protein T – S, where T20 of the native enzyme is S49 of the fusion protein
Protease Cleavage:
rTEV (ENLYFQG) residues 18 - 24
Cloning Sites:
EcoR1 site of pFastBAC HTa

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