Recombinant Protein - MAPK3 (ERK1) (2 - 379)

Name: MAPK3 (ERK1) (2 - 379)
Price per aliquot (100µg): £110.00
Catalog Number (DU Number):
DU1509
Accession:
BC013992
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH Sepharose
Enzymatic Assay Format:
Standard filter binding assay
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1 % 2-mercaptoethanol, 0.1 mM EGTA, 10 mM MgAc
Enzymatic Substrate:
Myelin basic protein Final concentration: 0.3 mg/ml
Calculated molecular mass:
Mono-Isotopic Mass: 69, 757.66 daltons
Average Mass: 69, 802.46 daltons
Purity:
>90 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 50 % glycerol, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine.
Storage Temperature:
-20 °C
Theoretical pI:
6.06
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids A2 – P379 (end) of human p44MAPK. Residue A232 of the fusion protein is equivalent to A2 of the native enzyme. The GST tag is located at residues 1 – 220. The following amino acid substitution is present: I – S, where I174 of the native sequence is S404 of the fusion protein
Protease Cleavage:
PreScission (LEVLFQGPL) residues 221 - 229
Cloning Sites:
BamH1 and Sal1 of pGEX6P-1

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