Recombinant Protein - Lck (2 - 509)

Name: Lck (2 - 509)
Price per aliquot (100µg): £110.00
Catalog Number (DU Number):
DU567
Accession:
X03533
Expression:
baculovirus
Tag and Terminus:
N-Term
6His
Uncleaved
Purification Method:
Ni2+-NTA agarose
Enzymatic Assay Format:
Standard filter binding assay
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1 % 2−mercaptoethanol, 0.1 mM EGTA, 10 mM MgAc
Enzymatic Substrate:
KVEKIGEGTYGVVYK [Residues 5 – 20 of human CDK2] Final concentration: 250 μM
Calculated molecular mass:
Mono-Isotopic Mass: 61, 796.44 daltons
Average Mass: 61, 835.76 daltons
Protein Activity:
Constitutively Active
Purity:
>85 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 50 % glycerol, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine.
Storage Temperature:
-20 °C
Theoretical PI:
5.16
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids G2 – P509 (end) of mouse Lck. Residue G31 of the fusion protein is equivalent to G2 of mouse Lck. The His(6) tag is located at residues 5 – 10. The following sequence is present after the Lck sequence, VDL, residues 539 – 541. The following amino acid substitutions are present: V – G, where V4 of the native sequence is G33 of the fusion protein. N – H, where N8 of the native sequence is H37 of the fusion protein.
Protease Cleavage:
rTEV (ENLYFQG) residues 18 - 24
Cloning Sites:
EcoR1 and Not1 sites of pFastBAC HTa

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