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Recombinant Protein - SAPK1b (JNK3 alpha 1) (40 - 422)

Name: SAPK1b (JNK3 alpha 1) (40 - 422)
Price per aliquot (100µg): £110.00
Synonyms:
JNK3, MAPK10
Catalog Number (DU Number):
DU1511
Accession:
NM_002753
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved

C-Term
6His
Uncleaved
Purification Method:
GSH Sepharose
Enzymatic Assay Format:
Standard filter binding assay
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1 % 2−mercaptoethanol, 0.1 mM EGTA, 10 mM MgAc
Enzymatic Substrate:
GST-ATF2 [19 - 96] [DU 1787] Final concentration: 0.2 mg/ml
Calculated molecular mass:
Mono-Isotopic Mass: 71, 566.49 daltons
Average Mass: 71, 612.87 daltons
Purity:
>85 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 150 mM NaCl, 270 mM sucrose, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-70 °C
Theoretical PI:
6.75
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids S40 – Q422 (end) of human JNK3 alpha 1. Residue S232 of the fusion protein is equilivalent to S40 of the native enzyme. The GST tag is located at residues 1 – 220 and the His(6) tag is at residues 615 – 620.
Protease Cleavage:
PreScission site (LEVLFQGPL) residues 221 – 229
Cloning Sites:
BamH1 site of pGEX6P-1

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