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Recombinant Protein - SAPK1c (JNK1 alpha 1) (1 - 384) *

Name: SAPK1c (JNK1 alpha 1) (1 - 384) *
Price per aliquot (100µg): £110.00
Synonyms:
JNK1, MAPK8
Catalog Number (DU Number):
DU700
Accession:
L26318
Expression:
baculovirus
Tag and Terminus:
N-Term
6His
Uncleaved
Purification Method:
Ni2+-NTA agarose
Enzymatic Assay Format:
Standard filter binding assay
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1 % 2−mercaptoethanol, 0.1 mM EGTA, 10 mM MgAc
Enzymatic Substrate:
GST-ATF2 [19 – 96] [DU 1787]: Final concentration: 0.2 mg/ml
Calculated molecular mass:
Mono-Isotopic Mass: 45, 153.99 daltons
Average Mass: 45, 183.28 daltons
Purity:
>90 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine.
Storage Temperature:
-70 °C
Theoretical PI:
7.65
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids M1 – Q384 (end) of human JNK1 alpha 1. Residue M8 of the fusion protein is equilivalent to M1 of the native enzyme. The His(6) tag is located at residues 2 - 7.
Protease Cleavage:
None
Cloning Sites:
Nde1 and Xhol sites of modified pFastBAC 1

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