Recombinant Protein - IKK epsilon (1 - 716)

Name: IKK epsilon (1 - 716)
Price per aliquot (100µg): £110.00
Catalog Number (DU Number):
DU14231
Accession:
NM_014002
Expression:
baculovirus
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH Sepharose
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 10 mM DTT, 0.1 mM EGTA, 10 mM MgAc
Enzymatic Substrate:
Myelin Basic Protein Final concentration: 1 mg/ml
Calculated molecular mass:
Mono-Isotopic Mass: 107, 218.34 daltons
Average Mass: 107, 286.48 daltons
Purity:
85 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.03 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine
Storage Temperature:
–70 °C
Theoretical pI:
6.90
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids M1 – M716 of human IKK alpha. Residue M232 of the fusion protein is equivalent to M1 of the native enzyme. The GST tag is located at residues 1 - 220.
Protease Cleavage:
PreScission (LEVLFQGP) residues 221 - 228
Cloning Sites:
BamH1 and Not1 sites of pFB DUAL6P-2.

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