Recombinant Protein - IKK beta (1 -736)

Name: IKK beta (1 -736)
Price per aliquot (100µg): £110.00
Long Name:
inhibitor of nuclear factor kappa B kinase subunit beta
Synonyms:
IKK2, NFKBIKB, IKK-beta, IKKB
Catalog Number (DU Number):
DU3167
Accession:
O14920-1
Expression:
baculovirus
Tag and Terminus:
N-Term
6His
Uncleaved
Purification Method:
Ni2+-NTA agarose
Enzymatic Assay Format:
Standard filter binding assay
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1mM EGTA, 0.1 % 2-mercaptoethanol, 10 mM MgAc
Enzymatic Substrate:
LDDRHDSGLDSMKDEEY Residues 26 – 42 of IkappaB alpha Final concentration: 30 μM
Calculated molecular mass:
Mono-Isotopic Mass: 85103.24 daltons
Average Mass: 85157.44 daltons
Protein Activity:
Constitutively Active
Purity:
85 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine.
Storage Temperature:
-70 °C
Theoretical PI:
6.12
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids M1 – P736 of human IKK beta (end residue S756). Residue M8 of fusion protein is equivalent to M1 of the native enzyme. The His(6) tag is located at residues 2 – 7.
Protease Cleavage:
None
Cloning Sites:
Nde1 and Not1 site of pFastBAC modified

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