Recombinant Protein - HIPK3 (161 - 562)

Name: HIPK3 (161 - 562)
Price per aliquot (100µg): £110.00
Catalog Number (DU Number):
DU5525
Accession:
NM_005734
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH Sepharose
Enzymatic Assay Format:
Standard filter binding assay
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1 mM EGTA, 0.1 % 2−mercaptoethanol, 10 mM MgAc
Enzymatic Substrate:
Myelin basic protein Final concentration: 0.3 mg/ml
Calculated molecular mass:
Mono-Isotopic Mass: 72, 983.07 daltons
Average Mass: 73, 030.34 daltons
Purity:
85 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-70 °C
Theoretical PI:
6.36
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids P161 – N562 of human HIPK3. [Full length protein ends at residue Y1214] Residue P235 of the fusion protein is equivalent to P161 of the native enzyme. The GST tag is located at residues 1 – 220.
Protease Cleavage:
PreScission (LEVLFQGPL) residues 221 - 229
Cloning Sites:
EcoR1 sites of pGEX 6P-1

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