Recombinant Protein - HIPK1 (158 - 555)

Name: HIPK1 (158 - 555)
Price per aliquot (100µg): £110.00
Catalog Number (DU Number):
DU5523
Accession:
NM_198268
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH Sepharose
Enzymatic Assay Format:
Standard filter binding assay
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1 mM EGTA, 0.1 % 2−mercaptoethanol, 10 mM MgAc
Enzymatic Substrate:
Myelin basic protein Final concentration: 0.3 mg/ml
Calculated molecular mass:
Mono-Isotopic Mass: 72, 954.03 daltons
Average Mass: 73, 001.33 daltons
Purity:
85 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-70 °C
Theoretical pI:
6.54
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids T158 – I555 of human HIPK1. [Full length protein ends at residue L1210] Residue T235 of the fusion protein is equivalent to T158 of the native enzyme. The GST tag is located at residues 1 – 220.
Protease Cleavage:
PreScission (LEVLFQGPL) residues 221 - 229
Cloning Sites:
EcoR1 sites of pGEX 6P-1

Do you need any help? Please get in touch and we’ll be happy to lend a hand.