50 mM Tris-HCl pH 7.5, 0.1 mM EGTA, 0.1 % 2−mercaptoethanol, 10 mM MgAc
Myelin basic protein Final concentration: 0.3 mg/ml
Calculated molecular mass:
Mono-Isotopic Mass: 72, 954.03 daltons
Average Mass: 73, 001.33 daltons
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Gel Information :
Gel Image 1:
Amino acids T158 – I555 of human HIPK1. [Full length protein ends at residue L1210]
Residue T235 of the fusion protein is equivalent to T158 of the native enzyme. The GST tag is located at residues 1 – 220.
PreScission (LEVLFQGPL) residues 221 - 229
EcoR1 sites of pGEX 6P-1
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