Recombinant Protein - GSK3 beta (2 - 420) *

Name: GSK3 beta (2 - 420) *
Price per aliquot (100µg): £110.00
Synonyms:
GSK3B
Catalog Number (DU Number):
DU899
Accession:
L33801
Expression:
baculovirus
Tag and Terminus:
N-Term
6His
Uncleaved

N-Term
EE
Uncleaved
Purification Method:
Ni2+-NTA agarose
Enzymatic Assay Format:
Standard filter binding assay
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1 % 2−mercaptoethanol, 0.1 mM EGTA, 10 mM MgAc
Enzymatic Substrate:
Phospho GS [YRRAAVPPSPSLSRHSSPHQS(PO4)EDEEE] Final concentration: 30 μM
Calculated molecular mass:
Mono-Isotopic Mass: 51, 020.72 daltons
Average Mass: 51, 053.06 daltons
Protein Activity:
Constitutively Active
Purity:
>85 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 50 % glycerol, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine.
Storage Temperature:
-20 °C
Theoretical pI:
8.43
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids S2 – T420 (end) of human GSK3 beta. Residue S38 of the fusion protein is equivalent to S2 of the native enzyme. The His(6) tag is located at residues 5 - 10 and the EE tag at residues 32 - 37. The following amino acid substitution is present: H – L, where H350 of the native sequence is L386 of the fusion protein
Protease Cleavage:
rTEV (ENLYFQG) residues 18 - 24
Cloning Sites:
BamH1/BglII and Not1 of pFastBAC HTb

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