Recombinant Protein - ERK8 (2 - 544)

Name: ERK8 (2 - 544)
Price per aliquot (100µg): £110.00
Synonyms:
MAPK15
Catalog Number (DU Number):
DU662
Accession:
AY065978
Expression:
baculovirus
Tag and Terminus:
N-Term
6His
Uncleaved
Purification Method:
Ni2+-NTA agarose
Enzymatic Assay Format:
Standard filter binding assay
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1 % 2−mercaptoethanol, 0.1 mM EGTA, 10 mM MgAc
Enzymatic Substrate:
Myelin basic protein Final concentration: 0.3 mg/ml
Calculated molecular mass:
Mono-Isotopic Mass: 62, 620.38 daltons
Average Mass: 62, 659.22 daltons
Protein Activity:
Constitutively Active
Purity:
75 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 50 % glycerol, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine.
Storage Temperature:
-20 °C
Theoretical PI:
8.84
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids C2 – V544 (end) of human ERK8. Residue C26 of the fusion protein is equilivalent to C2 of the native enzyme. The His(6) tag is located at residues 2 – 7 of the fusion protein.
Protease Cleavage:
rTEV (ENLYFQG) residues 15 - 21
Cloning Sites:
BamH1 and EcoR1 site of pFastBAC HTb

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