Recombinant Protein - ERBB4 (706 - 991)

Name: ERBB4 (706 - 991)
Price per aliquot (100µg): £110.00
Catalog Number (DU Number):
DU9180
Accession:
NM_005235
Expression:
baculovirus
Tag and Terminus:
N-Term
6His
Uncleaved
Purification Method:
Ni2+-NTA agarose
Enzymatic Assay Format:
Standard filter binding assay
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1mM EGTA, 0.1 % 2-mercaptoethanol, 10 mM MgAc
Enzymatic Substrate:
Poly Glu Tyr (4:1) Final concentration: 1 mg/ml
Calculated molecular mass:
Mono-Isotopic Mass: 35, 963.22 daltons
Average Mass: 35, 986.74 daltons
Protein Activity:
Constitutively Active
Purity:
75 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine.
Storage Temperature:
-70 °C [Long term stability to be determined]
Theoretical PI:
6.31
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids N706 – D991 of human ERBB4. [Full length protein ends at residue V1308] Residue N29 of fusion protein is equilivalent to N706 of the native enzyme. The His(6) tag is located at residues 5 – 10.
Protease Cleavage:
rTEV (ENLYFQG) residues 18 - 24
Cloning Sites:
BamH1 and HindIII site of pFastBAC HTb

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