50 mM Tris-HCl pH 7.5, 0.1mM EGTA, 0.1 % 2-mercaptoethanol, 10 mM MgAc
Poly Glu Tyr (4:1) Final concentration: 1 mg/ml
Calculated molecular mass:
Mono-Isotopic Mass: 35, 963.22 daltons
Average Mass: 35, 986.74 daltons
50 mM Tris-HCl pH 7.5, 270 mM sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine.
-70 °C [Long term stability to be determined]
Gel Information :
Gel Image 1:
Amino acids N706 – D991 of human ERBB4.
[Full length protein ends at residue V1308]
Residue N29 of fusion protein is equilivalent to N706 of the native enzyme. The His(6) tag is located at residues 5 – 10.
rTEV (ENLYFQG) residues 18 - 24
BamH1 and HindIII site of pFastBAC HTb
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