Recombinant Protein - EPH B3 (561 - 998)

Name: EPH B3 (561 - 998)
Price per aliquot (100µg): £110.00
Catalog Number (DU Number):
DU4875
Accession:
NM_004443
Expression:
baculovirus
Tag and Terminus:
N-Term
6His
Uncleaved
Purification Method:
Ni2+-NTA agarose
Enzymatic Assay Format:
Standard filter binding assay
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1 mM EGTA, 0.1 % 2−mercaptoethanol, 10 mM MgAc
Enzymatic Substrate:
Poly Glu Tyr (4:1) Final concentration: 0.1 mg/ml
Calculated molecular mass:
Mono-Isotopic Mass: 52, 528.45 daltons
Average Mass: 52, 562.31 daltons
Protein Activity:
Constitutively Active
Purity:
80 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-80 °C
Theoretical PI:
6.03
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids V561 – V998 (end) of human EPH B3. Residue V29 of the fusion protein is equivalent to V561 of the native enzyme. The His(6) tag is located at residues 5 – 10.
Protease Cleavage:
rTEV (ENLYFQG) residues 18 - 24
Cloning Sites:
BamH1 and Not1 sites of pFastBAC HTb

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