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Recombinant Protein - EPH A2 (591 - 976)
Name: EPH A2 (591 - 976)
Price per aliquot (100µg): £100.00
EPH receptor A2
Catalog Number (DU Number):
Enzymatic Assay Format:
Standard filter binding assay
50 mM Tris-HCl pH 7.5, 0.1 mM EGTA, 0.1 % 2−mercaptoethanol, 10 mM MgAc
Poly Glu Tyr (4:1) Final concentration: 0.1 mg/ml
Calculated molecular mass:
Mono-Isotopic Mass: 70, 354.92 daltons
Average Mass: 70, 400.53 daltons
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Amino acids P591 – I976 (end) of human EPH A2.
Residue P232 of the fusion protein is equivalent to P591 of the native enzyme. The GST tag is located at residues 1 – 220.
PreScission (LEVLFQGPL) residues 221 - 229
BglIII and Sal1site of pGEX 6P-1
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