FESTIVE CLOSURE

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Recombinant Protein - EF2 Kinase (2 - 725)

Name: EF2 Kinase (2 - 725)
Price per aliquot (100µg): £100.00
Catalog Number (DU Number):
DU1446
Accession:
AAH32665
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH Sepharose
Enzymatic Assay Format:
Standard filter binding assay
Enzymatic Buffer:
50 mM Hepes pH 6.6, 200 μM CaCl2, 0.3 μM calmodulin, 0.1 % 2 mercaptoethanol, 10 mM magnesium acetate
Enzymatic Substrate:
RKKFGESEKTKTKEFL [Residues 2020 – 2035 of Dictyostelium myosin heavy chains] Final concentration: 300 μM
Calculated molecular mass:
Mono-Isotopic Mass: 108, 283.87 daltons
Average Mass: 108, 352.58 daltons
Protein Activity:
Constitutively Active
Purity:
90 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-70 °C
Theoretical pI:
5.32
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids A2 – E725 (end) of human EF2 kinase. Residue A227 of the fusion protein is equivalent to A2 of the native enzyme. The GST tag is located at residues 1 - 220.
Protease Cleavage:
Thrombin (LVPRGS) residues 221 - 226
Cloning Sites:
BamH1 and EcoR1 site of pGEX 4T-1

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