Recombinant Protein - DYRK3 (1 - 588)

Name: DYRK3 (1 - 588)
Price per aliquot (100µg): £100.00
Catalog Number (DU Number):
DU3371
Accession:
AY590695
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH Sepharose
Enzymatic Assay Format:
Standard filter binding assay
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1 % 2−mercaptoethanol, 0.1 mM EGTA, 10 mM MgAc
Calculated molecular mass:
Mono-Isotopic Mass: 92, 478.50 daltons
Average Mass: 92, 537.90 daltons
Protein Activity:
Constitutively Active
Purity:
>75 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine
Storage Temperature:
-70 °C
Theoretical pI:
9.02
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids M1 – S588 (end) of human DYRK3. Residue M232 of the fusion protein is equilivalent to M1 of the native enzyme. The GST tag is located at residues 1 - 220.
Protease Cleavage:
PreScission (LEVLFQGPL) residues 221 - 229
Cloning Sites:
BamH1 and Not1 site of pGEX6P-1

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