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Recombinant Protein - DYRK2 (3 - 528)
Name: DYRK2 (3 - 528)
Price per aliquot (100µg): £100.00
Catalog Number (DU Number):
Enzymatic Assay Format:
Standard filter binding assay
50 mM Tris-HCl pH 7.5, 0.1 % 2mercaptoethanol, 0.1 mM EGTA,10 mM MgAc
WOODtide [KKISGRLSPIMTEQ] Final concentration: 500 μM
Calculated molecular mass:
Mono-Isotopic Mass: 86, 533.48 daltons
Average Mass: 86, 589 daltons
50 mM Tris-HCl pH 7.5, 50 % glycerol, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine
Amino acids D3 – S528 (end) of human DYRK2.
Residue D235 of the fusion protein is equilivalent to D3 of the native enzyme. The GST tag is located at residues 1 - 220.
Thrombin (LVPRGS) at residues 221 – 226
BamH1 and EcoR1 site of pGEX4T-1
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