FESTIVE CLOSURE

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Recombinant Protein - DYRK1a (1 - 499)

Name: DYRK1a (1 - 499)
Price per aliquot (100µg): £100.00
Catalog Number (DU Number):
DU1501
Accession:
X79769
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH Sepharose
Enzymatic Assay Format:
Standard filter binding assay
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1 % 2mercaptoethanol, 0.1 mM EGTA, 10 mM MgAc
Enzymatic Substrate:
WOODtide [KKISGRLSPIMTEQ] Final concentration: 500 μM
Calculated molecular mass:
Mono-Isotopic Mass: 85, 087.39 daltons
Average Mass: 85, 142.12 daltons
Protein Activity:
Constitutively Active
Purity:
>70 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 50 % glycerol, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine
Storage Temperature:
-20 °C
Theoretical pI:
8.94
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids M1 – L499 of rat DYRK1a. [Full length protein ends at residue S763] Residue M239 of the fusion protein is equilivalent to M1 of the native enzyme. The GST tag is located at residues 1 - 220 of the fusion protein.
Protease Cleavage:
Thrombin (LVPRGS) at residues 221 – 226
Cloning Sites:
BamH1 site of pGEX-4T1

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