50 mM Tris-HCl pH 7.5, 0.1 % 2mercaptoethanol, 0.1 mM EGTA, 10 mM MgAc
WOODtide [KKISGRLSPIMTEQ] Final concentration: 500 μM
Calculated molecular mass:
Mono-Isotopic Mass: 85, 087.39 daltons
Average Mass: 85, 142.12 daltons
50 mM Tris-HCl pH 7.5, 50 % glycerol, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine
Gel Information :
Gel Image 1:
Amino acids M1 – L499 of rat DYRK1a.
[Full length protein ends at residue S763]
Residue M239 of the fusion protein is equilivalent to M1 of the native enzyme. The GST tag is located at residues 1 - 220 of the fusion protein.
Thrombin (LVPRGS) at residues 221 – 226
BamH1 site of pGEX-4T1
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