Name: TPL2 (COT) (30 - 397)
Price per aliquot (100µg): £110.00
Catalog Number (DU Number):
Enzymatic Assay Format:
Three step assay in which COT activates unactive MKK1 [DU 1843], which in turn activates unactive p42MAPKinase [DU 650 or DU 1844]., Activity of p42MAPkinase is then assayed against myelin basic protein as substrate (final concentration of 0.3 mg/ml), in the standard filter binding assay.
50 mM Tris-HCl pH 7.5, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.2 mM sodium vanadate, 0.5 μM microcystin-LR, 10 mM magnesium acetate
Calculated molecular mass:
Mono-Isotopic Mass: 45, 146.56 daltons
Average Mass: 45, 175.56 daltons
50 mM Tris-HCl pH 7.5, 270 mM sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine.
-70 °C [Long term stability to be determined]
Amino acids M30 – R397 of human COT.
[Full length native protein ends at residue G467]
Residue M29 of fusion protein is equilivalent to M30 of the native enzyme. The His(6) tag is located at residues 5 – 10.
rTEV (ENLYFQG) residues 18 - 24
BamH1 and EcoR1 site of pFastBAC HTb