Recombinant Protein - TPL2 (COT) (30 - 397)

Name: TPL2 (COT) (30 - 397)
Price per aliquot (100µg): £110.00
Synonyms:
MAP3K8
Catalog Number (DU Number):
DU1810
Accession:
P41279
Expression:
baculovirus
Tag and Terminus:
N-Term
6His
Uncleaved
Purification Method:
Ni2+-NTA agarose
Enzymatic Assay Format:
Three step assay in which COT activates unactive MKK1 [DU 1843], which in turn activates unactive p42MAPKinase [DU 650 or DU 1844]., Activity of p42MAPkinase is then assayed against myelin basic protein as substrate (final concentration of 0.3 mg/ml), in the standard filter binding assay.
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.2 mM sodium vanadate, 0.5 μM microcystin-LR, 10 mM magnesium acetate
Calculated molecular mass:
Mono-Isotopic Mass: 45, 146.56 daltons
Average Mass: 45, 175.56 daltons
Protein Activity:
Constitutively Active
Purity:
75 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine.
Storage Temperature:
-70 °C [Long term stability to be determined]
Theoretical pI:
6.01
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids M30 – R397 of human COT. [Full length native protein ends at residue G467] Residue M29 of fusion protein is equilivalent to M30 of the native enzyme. The His(6) tag is located at residues 5 – 10.
Protease Cleavage:
rTEV (ENLYFQG) residues 18 - 24
Cloning Sites:
BamH1 and EcoR1 site of pFastBAC HTb

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