Recombinant Protein - CK2 alpha 1 (2 - 391)

Name: CK2 alpha 1 (2 - 391)
Price per aliquot (100µg): £100.00
Long Name:
Casein Kinase 2 alpha 1 [2 - 391]
Catalog Number (DU Number):
DU813
Accession:
NM_001895
Expression:
baculovirus
Tag and Terminus:
N-Term
6His
Uncleaved
Purification Method:
Ni2+-NTA agarose
Enzymatic Assay Format:
Standard filter binding assay
Enzymatic Buffer:
20 mM Hepes pH 7.5, 5 mM DTT, 0.1 mM EDTA, 150 mM NaCl, 0.1 % Triton X-100, 10 mM MgAc
Enzymatic Substrate:
CK2 substrate peptide [RRRDDDSDDD] Final concentration: 300 μM
Calculated molecular mass:
Mono-Isotopic Mass: 48, 353.17 daltons
Average Mass: 48, 384.01 daltons
Protein Activity:
Constitutively Active
Purity:
>80 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.2 mM PMSF, 1 mM Benzamidine.
Storage Temperature:
-70 °C [Long term stability to be determined]
Theoretical pI:
6.75
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids S2 – Q391 (end) of human CK2 alpha 1. Residue S29 of the fusion protein is equivalent to S2 of the native enzyme. The His(6) tag is located at residues 5 – 10.
Protease Cleavage:
rTEV (ENLYFQG) residues 18 - 24
Cloning Sites:
BamH1 and EcoR1 sites of pFastBAC HTb

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