FESTIVE CLOSURE

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Recombinant Protein - CHK2 (5 - 543)

Name: CHK2 (5 - 543)
Price per aliquot (100µg): £100.00
Synonyms:
CHEK2
Catalog Number (DU Number):
DU1663
Accession:
NM_007194
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved

C-Term
6His
Uncleaved
Purification Method:
GSH Sepharose followed by Ni2+-NTA agarose
Enzymatic Assay Format:
Standard filter binding assay
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1 % 2 mercaptoethanol, 0.1 mM EGTA, 10 mM MgAc
Enzymatic Substrate:
CHKtide [KKKVSRSGLYRSPSMPENLNRPR]; Final concentration: 250 μM
Calculated molecular mass:
Mono-Isotopic Mass: 89,430.11
Average Mass: 89,486.98
Protein Activity:
Constitutively Active
Purity:
>80 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine.
Storage Temperature:
-70 °C
Theoretical pI:
6.28
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids S5 – L543 (end) of human CHK2. Residue S244 of the fusion protein is equilivalent to S5 of the native enzyme. The GST tag is located at residues 1 - 220 and the C-terminal His(6) tag at residues 783 - 788.
Protease Cleavage:
Thrombin (LVPRGS) at residues 221 - 226.
Cloning Sites:
Nde1 and EcoR1 sites of modified pGEX-2TK

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