50 mM Tris-HCl pH 7.5, 0.1 % 2 mercaptoethanol, 0.1 mM EGTA, 10 mM MgAc
CHKtide [KKKVSRSGLYRSPSMPENLNRPR]; Final concentration: 250 μM
Calculated molecular mass:
Mono-Isotopic Mass: 89,430.11
Average Mass: 89,486.98
50 mM Tris-HCl pH 7.5, 270 mM sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine.
Gel Information :
Gel Image 1:
Amino acids S5 – L543 (end) of human CHK2. Residue S244 of the fusion protein is equilivalent to S5 of the native enzyme. The GST tag is located at residues 1 - 220 and the C-terminal His(6) tag at residues 783 - 788.
Thrombin (LVPRGS) at residues 221 - 226.
Nde1 and EcoR1 sites of modified pGEX-2TK
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