FESTIVE CLOSURE

Festive Closure

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Recombinant Protein - CHK1 (1 - 476)

Name: CHK1 (1 - 476)
Price per aliquot (100µg): £100.00
Synonyms:
CHEK1
Catalog Number (DU Number):
DU578
Accession:
AF016582
Expression:
baculovirus
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH Sepharose
Enzymatic Assay Format:
Standard filter binding assay
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1 % 2 mercaptoethanol, 0.1 mM EGTA, 0.02 % Brij-35, 10 mM magnesium acetate
Enzymatic Substrate:
CHKtide [KKKVSRSGLYRSPSMPENLNRPR]; Final concentration: 30 μM
Calculated molecular mass:
Mono-Isotopic Mass: 81,564.69
Average Mass: 81,617.2
Protein Activity:
Constitutively Active
Purity:
>80 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35
Storage Temperature:
-70 °C
Theoretical pI:
6.97
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids M1 – T476 (end) of human CHK1. ResidueM235 of the fusion protein is equivalent to M1 of the native enzyme. The GST tag is located between residues 1 - 220 of the fusion protein.
Protease Cleavage:
PreScission site (LEVLFQGPL) residues 221-229
Cloning Sites:
EcoR1 and Not1 of pFastBac GST

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