FESTIVE CLOSURE

Festive Closure

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Recombinant Protein - CaMKK alpha (1 - 505)

Name: CaMKK alpha (1 - 505)
Price per aliquot (100µg): £100.00
Long Name:
CaMKK alpha isoform a
Catalog Number (DU Number):
DU8208
Accession:
NM_172206
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved

C-Term
6His
Uncleaved
Purification Method:
GSH Sepharose
Enzymatic Assay Format:
Standard filter binding assay
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 500 μM CaCl2, 0.3 μM calmodulin, 0.1 mM EGTA, 0.1 % 2−mercaptoethanol, 10 mM magnesium acetate
Enzymatic Substrate:
AKPKGNKDYHLQTCCGSLAYRRR, residues 155 – 175 of human MELK (T loop + added Arg residues at C terminus) Final concentration: 300 μM
Calculated molecular mass:
Mono-Isotopic Mass: 83,686.83
Average Mass: 83,739.84
Protein Activity:
Constitutively Active
Purity:
85 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-20 °C
Theoretical pI:
5.75
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids M1 – S505 (end) of human CaMKK alpha isoform a. Residue M235 of the fusion protein is equivalent to M1 of the native enzyme. The GST tag is located at residues 1 – 220 and the His(6) tag is located at residues 740 – 745.
Protease Cleavage:
PreScission (LEVLFQGPL) residues 221 - 229
Cloning Sites:
EcoR1 and Not1site of pGEX 6P-1

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